2ugi
PROTEIN MIMICRY OF DNA FROM CRYSTAL STRUCTURES OF THE URACIL GLYCOSYLASE INHIBITOR PROTEIN AND ITS COMPLEX WITH ESCHERICHIA COLI URACIL-DNA GLYCOSYLASE
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OverviewOverview
Uracil-DNA glycosylase (UDG), which is a critical enzyme in DNA, base-excision repair that recognizes and removes uracil from DNA, is, specifically and irreversably inhibited by the thermostable uracil-DNA, glycosylase inhibitor protein (Ugi). A paradox for the highly specific Ugi, inhibition of UDG is how Ugi can successfully mimic DNA backbone, interactions for UDG without resulting in significant cross-reactivity, with numerous other enzymes that possess DNA backbone binding affinity., High-resolution X-ray crystal structures of Ugi both free and in complex, with wild-type and the functionally defective His187Asp mutant Escherichia, coli UDGs reveal the detailed molecular basis for duplex DNA backbone, mimicry by Ugi. The overall shape and charge distribution of Ugi most, closely resembles a midpoint in a trajectory between B-form DNA and the, kinked DNA observed in UDG:DNA product complexes. Thus, Ugi targets the, mechanism of uracil flipping by UDG and appears to be a transition-state, mimic for UDG-flipping of uracil nucleotides from DNA. Essentially all the, exquisite shape, electrostatic and hydrophobic complementarity for the, high-affinity UDG-Ugi interaction is pre-existing, except for a key flip, of the Ugi Gln19 carbonyl group and Glu20 side-chain, which is triggered, by the formation of the complex. Conformational changes between unbound, Ugi and Ugi complexed with UDG involve the beta-zipper structural motif, which we have named for the reversible pairing observed between, intramolecular beta-strands. A similar beta-zipper is observed in the, conversion between the open and closed forms of UDG. The combination of, extremely high levels of pre-existing structural complementarity to DNA, binding features specific to UDG with key local conformational changes in, Ugi resolves the UDG-Ugi paradox and suggests a potentially general, structural solution to the formation of very high affinity DNA, enzyme-inhibitor complexes that avoid cross- reactivity.
About this StructureAbout this Structure
2UGI is a Single protein structure of sequence from Phage pbs1 with IMD as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Protein mimicry of DNA from crystal structures of the uracil-DNA glycosylase inhibitor protein and its complex with Escherichia coli uracil-DNA glycosylase., Putnam CD, Shroyer MJ, Lundquist AJ, Mol CD, Arvai AS, Mosbaugh DW, Tainer JA, J Mol Biol. 1999 Mar 26;287(2):331-46. PMID:10080896
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