2rmc
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CRYSTAL STRUCTURE OF MURINE CYCLOPHILIN C COMPLEXED WITH IMMUNOSUPPRESSIVE DRUG CYCLOSPORIN A
OverviewOverview
Cyclophilin is a cellular receptor for the immunosuppressive drug, cyclosporin A (CsA). Cyclophilin C (CyPC) is highly expressed in murine, kidney, making it a potential mediator of the nephrotoxic effects of CsA., The structure of murine CyPC complexed with CsA has been solved and, refined to an R factor of 0.197 at a 1.64-A resolution. Superposition of, the CyPC-CsA structure with the unligated cyclophilin A (CyPA) revealed, significant migration of three loops: Gln-179 to Thr-189, Asp-47 to, Lys-49, and Met-170 to Ile-176. The proximity of the loop Gln-179 to, Thr-189 to the CsA binding site may account for the unique binding of a, 77-kDa glycoprotein, CyPC binding protein (CyCAP), to CyPC. The binding of, CsA to CyPC is similar to that of CsA to human T-cell cyclophilin A, (CyPA). However, the conformation of CsA when bound to CyPC is, significantly different from that when bound to CyPA. These differences, may reflect conformational variation of CsA when bound to different, proteins. Alternatively, the previous CyPA-CsA structure at low resolution, may not provide sufficient details for a comparison with the CyPC-CsA, structure.
About this StructureAbout this Structure
2RMC is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of murine cyclophilin C complexed with immunosuppressive drug cyclosporin A., Ke H, Zhao Y, Luo F, Weissman I, Friedman J, Proc Natl Acad Sci U S A. 1993 Dec 15;90(24):11850-4. PMID:8265636
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