2px8
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Crystal structure of the Murray Valley Encephalitis Virus NS5 2'-O Methyltransferase domain in complex with SAH and 7M-GTP
OverviewOverview
We have determined the high resolution crystal structure of the, methyltransferase domain of the NS5 polypeptide from the Murray Valley, encephalitis virus. This domain is unusual in having both the N7 and 2'-O, methyltransferase activity required for Cap 1 synthesis. We have also, determined structures for complexes of this domain with nucleotides and, cap analogues providing information on cap binding, based on which we, suggest a model of how the sequential methylation of the N7 and 2'-O, groups of the cap may be coordinated.
About this StructureAbout this Structure
2PX8 is a Single protein structure of sequence from Murray valley encephalitis virus with CL, MGT, 3PO, SAH and GOL as ligands. Active as RNA-directed RNA polymerase, with EC number 2.7.7.48 Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the Murray Valley encephalitis virus NS5 methyltransferase domain in complex with cap analogues., Assenberg R, Ren J, Verma A, Walter TS, Alderton D, Hurrelbrink RJ, Fuller SD, Bressanelli S, Owens RJ, Stuart DI, Grimes JM, J Gen Virol. 2007 Aug;88(Pt 8):2228-36. PMID:17622627
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- Murray valley encephalitis virus
- RNA-directed RNA polymerase
- Single protein
- Alderton, D.
- Assenberg, R.
- Fuller, S.D.
- Grimes, J.M.
- Hurrelbrink, R.J.
- OPPF, Oxford.Protein.Production.Facility.
- Owens, R.J.
- Ren, J.
- Stuart, D.I.
- Verma, A.
- Walter, T.S.
- 3PO
- CL
- GOL
- MGT
- SAH
- 3po
- 7m-gtp
- Methyltransferase
- Oppf
- Oxford protein production facility
- Sah
- Structural genomics