2np2

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Revision as of 13:42, 21 November 2007 by OCA (talk | contribs) (New page: left|200px<br /><applet load="2np2" size="450" color="white" frame="true" align="right" spinBox="true" caption="2np2, resolution 3.02Å" /> '''Hbb-DNA complex'''<b...)
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File:2np2.gif


2np2, resolution 3.02Å

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Hbb-DNA complex

OverviewOverview

The genome of the Lyme disease-causing spirochete Borrelia burgdorferi, encodes only a single polypeptide from the integration host factor, (IHF)/HU or 'DNABII' family of nucleoid-associated proteins - Hbb. DNABII, proteins induce large bends in DNA and serve as architectural factors in a, variety of prokaryotic cellular processes. We have solved the crystal, structure of an Hbb-DNA complex in which the DNA is bent by over 180, degrees . We find that like IHF, Hbb relies exclusively on indirect, readout to recognize its cognate site. Additional binding studies show, that the sequence preferences of Hbb are related to, yet distinct from, those of IHF. Defining these binding characteristics may help to uncover, additional roles for Hbb in Borrelia DNA metabolism as well as further our, understanding of the mechanism of indirect readout.

About this StructureAbout this Structure

2NP2 is a Single protein structure of sequence from Borrelia burgdorferi. Full crystallographic information is available from OCA.

ReferenceReference

Shaping the Borrelia burgdorferi genome: crystal structure and binding properties of the DNA-bending protein Hbb., Mouw KW, Rice PA, Mol Microbiol. 2007 Mar;63(5):1319-30. Epub 2007 Jan 22. PMID:17244195

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