2jxr
STRUCTURE OF YEAST PROTEINASE A
|
OverviewOverview
The crystal structures of glycosylated native proteinase A, an aspartic, proteinase found in the vacuole of Saccharomyces cerevisiae, and its, complex with a difluorostatone-containing tripeptide have been determined, by molecular replacement to 3.5 A and 2.4 A resolutions, respectively., Superposition of the bound and native forms gave an r.m.s. difference of, 0.6 A largely reflecting the poor resolution of the native crystal, structure. The secondary and tertiary structures are highly similar to, those found in porcine pepsin and lysosomal cathepsin D; superposition of, the structure of proteinase A bound to the difluorostatone inhibitor on, those of pepsin and cathepsin D gave pairwise r.m.s. differences for, C(alpha) atoms of 1.36 A and 0.88 A. Most differences occur in loop, regions. Comparison of the structure of the proteinase A-difluorostatone, complex with that of endothiapepsin bound with the same inhibitor shows, that the conformation and hydrogen bond interactions of the inhibitor in, the active site are very similar, even though the enzymes have only 27%, sequence identity. Electron density for the crystal structure of the, proteinase A complex reveals five residues of the oligosaccharide, structure attached to Asn67: Man-(1 --> 2)-alpha-Man-(1 --> 3)-beta-Man-(1, --> 4)-beta-GlcNAc-(1 --> 4)-beta-GlcNAc-Asn-67. The first three residues, of the oligosaccharide cover the same region of the protein surface as, those of the oligosaccharide attached to the equivalent position in, cathepsin D. The second carbohydrate attachment site is disordered beyond, the first carbohydrate residue in both enzymes.
About this StructureAbout this Structure
2JXR is a Single protein structure of sequence from Saccharomyces cerevisiae with NDG as ligand. This structure superseeds the now removed PDB entry 1JXR. Active as Saccharopepsin, with EC number 3.4.23.25 Full crystallographic information is available from OCA.
ReferenceReference
The three-dimensional structure at 2.4 A resolution of glycosylated proteinase A from the lysosome-like vacuole of Saccharomyces cerevisiae., Aguilar CF, Cronin NB, Badasso M, Dreyer T, Newman MP, Cooper JB, Hoover DJ, Wood SP, Johnson MS, Blundell TL, J Mol Biol. 1997 Apr 11;267(4):899-915. PMID:9135120
Page seeded by OCA on Wed Nov 21 12:40:36 2007