2cam
AVIDIN MUTANT (K3E,K9E,R26D,R124L)
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OverviewOverview
The mature hen avidin encoded by a synthetic cDNA was expressed in, Escherichia coli in an insoluble form. After resolubilization, renaturation and purification, a recovery of about 20 mg/l cell culture, was obtained. ELISA assays indicated no apparent differences in biotin, binding between the natural and recombinant avidins. In addition, an, acidic avidin mutant, bearing the substitutions Lys3-->Glu, Lys9--> Glu, Arg26-->Asp and Arg124-->Leu of four exposed basic residues, was produced., The protein, expressed and renatured as wild-type avidin, showed unaltered, biotin-binding activity. The acidic pI (approximately 5.5) and lack of, aggregation of the mutant allowed easy electrophoretic analysis under, non-denaturing conditions of the protein alone and of its complexes with, ... [(full description)]
About this StructureAbout this Structure
2CAM is a [Single protein] structure of sequence from [Gallus gallus]. Full crystallographic information is available from [OCA].
ReferenceReference
Biochemical characterization and crystal structure of a recombinant hen avidin and its acidic mutant expressed in Escherichia coli., Nardone E, Rosano C, Santambrogio P, Curnis F, Corti A, Magni F, Siccardi AG, Paganelli G, Losso R, Apreda B, Bolognesi M, Sidoli A, Arosio P, Eur J Biochem. 1998 Sep 1;256(2):453-60. PMID:9760187
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