2hvs
Structure of T4 RNA Ligase 2 with Nicked 5'-Adenylated nucleic acid duplex containing a 2'-deoxyribonucleotide at the nick
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OverviewOverview
T4 RNA ligase 2 (Rnl2) and kinetoplastid RNA editing ligases exemplify a, family of RNA repair enzymes that seal 3'OH/5'PO(4) nicks in duplex RNAs, via ligase adenylylation (step 1), AMP transfer to the nick 5'PO(4) (step, 2), and attack by the nick 3'OH on the 5'-adenylylated strand to form a, phosphodiester (step 3). Crystal structures are reported for Rnl2 at, discrete steps along this pathway: the covalent Rnl2-AMP intermediate;, Rnl2 bound to an adenylylated nicked duplex, captured immediately, following step 2; and Rnl2 at an adenylylated nick in a state poised for, step 3. These structures illuminate the stereochemistry of nucleotidyl, transfer and reveal how remodeling of active-site contacts and, conformational changes propel the ligation reaction forward. Mutational, analysis and comparison of nick-bound structures of Rnl2 and human DNA, ligase I highlight common and divergent themes of substrate recognition, that can explain their specialization for RNA versus DNA repair.
About this StructureAbout this Structure
2HVS is a Single protein structure of sequence from Bacteriophage t4 with BTB as ligand. Full crystallographic information is available from OCA.
ReferenceReference
RNA ligase structures reveal the basis for RNA specificity and conformational changes that drive ligation forward., Nandakumar J, Shuman S, Lima CD, Cell. 2006 Oct 6;127(1):71-84. PMID:17018278
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