2hpd
CRYSTAL STRUCTURE OF HEMOPROTEIN DOMAIN OF P450BM-3, A PROTOTYPE FOR MICROSOMAL P450'S
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OverviewOverview
Cytochrome P450BM-3, a bacterial fatty acid monoxygenase, resembles the, eukaryotic microsomal P450's and their flavoprotein reductase in primary, structure and function. The three-dimensional structure of the hemoprotein, domain of P450BM-3 was determined by x-ray diffraction and refined to an R, factor of 16.9 percent at 2.0 angstrom resolution. The structure consists, of an alph and a beta domain. The active site heme is accessible through a, long hydrophobic channel formed primarily by the beta domain and the B', and F helices of the alpha domain. The two molecules in the asymmetric, unit differ in conformation around the substrate binding pocket., Substantial differences between P450BM-3 and P450cam, the only other P450, structure available, are observed around the substrate binding pocket and, the regions important for redox partner binding. A general mechanism for, proton transfer in P450's is also proposed.
About this StructureAbout this Structure
2HPD is a Single protein structure of sequence from Bacillus megaterium with HEM as ligand. Active as Unspecific monooxygenase, with EC number 1.14.14.1 Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of hemoprotein domain of P450BM-3, a prototype for microsomal P450's., Ravichandran KG, Boddupalli SS, Hasermann CA, Peterson JA, Deisenhofer J, Science. 1993 Aug 6;261(5122):731-6. PMID:8342039
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