2h5a

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Revision as of 12:25, 21 November 2007 by OCA (talk | contribs) (New page: left|200px<br /><applet load="2h5a" size="450" color="white" frame="true" align="right" spinBox="true" caption="2h5a, resolution 1.72Å" /> '''Complex of the enzym...)
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File:2h5a.gif


2h5a, resolution 1.72Å

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Complex of the enzyme PMM/PGM with xylose 1-phosphate

OverviewOverview

Two complexes of the enzyme phosphomannomutase/phosphoglucomutase, (PMM/PGM) from Pseudomonas aeruginosa with a slow substrate and with an, inhibitor have been characterized by X-ray crystallography. Both ligands, induce an interdomain rearrangement in the enzyme that creates a highly, buried active site. Comparisons with enzyme-substrate complexes show that, the inhibitor xylose 1-phosphate utilizes many of the previously observed, enzyme-ligand interactions. In contrast, analysis of the ribose, 1-phosphate complex reveals a combination of new and conserved, enzyme-ligand interactions for binding. The ability of PMM/PGM to, accommodate these two pentose phosphosugars in its active site may be, relevant for future efforts towards inhibitor design.

About this StructureAbout this Structure

2H5A is a Single protein structure of sequence from Pseudomonas aeruginosa with ZN and X1P as ligands. Full crystallographic information is available from OCA.

ReferenceReference

Complexes of the enzyme phosphomannomutase/phosphoglucomutase with a slow substrate and an inhibitor., Regni C, Shackelford GS, Beamer LJ, Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006 Aug 1;62(Pt, 8):722-6. Epub 2006 Jul 24. PMID:16880541

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