2gn1
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Crystal structure of dimeric biodegradative threonine deaminase (TdcB) from Salmonella typhimurium at 2.2A resolution (Triclinic form with one dimer of TdcB in the asymmetric unit)
OverviewOverview
Two different pyridoxal 5'-phosphate-containing l-threonine deaminases (EC, 4.3.1.19), biosynthetic and biodegradative, which catalyze the deamination, of l-threonine to alpha-ketobutyrate, are present in Escherichia coli and, Salmonella typhimurium. Biodegradative threonine deaminase (TdcB), catalyzes the first reaction in the anaerobic breakdown of l-threonine to, propionate. TdcB, unlike the biosynthetic threonine deaminase, is, insensitive to l-isoleucine and is activated by AMP. In the present study, TdcB from S. typhimurium was cloned and overexpressed in E. coli. In the, presence of AMP or CMP, the recombinant enzyme was converted to the, tetrameric form accompanied by significant enzyme activation. To provide, insights into ligand-mediated oligomerization and enzyme activation, crystal structures of S. typhimurium TdcB and its complex with CMP were, determined. In the native structure, TdcB is in a dimeric form, whereas in, the TdcB.CMP complex, it exists in a tetrameric form with 222 symmetry and, appears as a dimer of dimers. Tetrameric TdcB binds to four molecules of, CMP, two at each of the dimer interfaces. Comparison of the dimer, structure in the ligand (CMP)-free and -bound forms suggests that the, changes induced by ligand binding at the dimer interface are essential for, tetramerization. The differences observed in the tertiary and quaternary, structures of TdcB in the absence and presence of CMP appear to account, for enzyme activation and increased binding affinity for l-threonine., Comparison of TdcB with related pyridoxal 5'-phosphate-dependent enzymes, points to structural and mechanistic similarities.
About this StructureAbout this Structure
2GN1 is a Single protein structure of sequence from Salmonella typhimurium with NA as ligand. Active as Threonine ammonia-lyase, with EC number 4.3.1.19 Full crystallographic information is available from OCA.
ReferenceReference
Crystal structures of Salmonella typhimurium biodegradative threonine deaminase and its complex with CMP provide structural insights into ligand-induced oligomerization and enzyme activation., Simanshu DK, Savithri HS, Murthy MR, J Biol Chem. 2006 Dec 22;281(51):39630-41. Epub 2006 Oct 17. PMID:17046821
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