2br0
DNA ADDUCT BYPASS POLYMERIZATION BY SULFOLOBUS SOLFATARICUS DPO4. ANALYSIS AND CRYSTAL STRUCTURES OF MULTIPLE BASE-PAIR SUBSTITUTION AND FRAMESHIFT PRODUCTS WITH THE ADDUCT 1,N2-ETHENOGUANINE
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OverviewOverview
1,N(2)-Etheno(epsilon)guanine is a mutagenic DNA lesion derived from lipid, oxidation products and also from some chemical carcinogens. Gel, electrophoretic analysis of the products of primer extension by Sulfolobus, solfataricus P2 DNA polymerase IV (Dpo4) indicated preferential, incorporation of A opposite 3'-(1,N(2)-epsilon-G)TACT-5', among the four, dNTPs tested individually. With the template 3'-(1,N(2)-epsilon-G)CACT-5', both G and A were incorporated. When primer extension was done in the, presence of a mixture of all four dNTPs, high pressure liquid, chromatography-mass spectrometry analysis of the products indicated that, (opposite 3'-(1,N(2)-epsilon-G)CACT-5') the major product was 5'-GTGA-3', and the minor product was 5'-AGTGA-3'. With the template, ... [(full description)]
About this StructureAbout this Structure
2BR0 is a [Single protein] structure of sequence from [Sulfolobus solfataricus] with CA and DG3 as [ligands]. Active as [[1]], with EC number [2.7.7.7]. Full crystallographic information is available from [OCA].
ReferenceReference
DNA adduct bypass polymerization by Sulfolobus solfataricus DNA polymerase Dpo4: analysis and crystal structures of multiple base pair substitution and frameshift products with the adduct 1,N2-ethenoguanine., Zang H, Goodenough AK, Choi JY, Irimia A, Loukachevitch LV, Kozekov ID, Angel KC, Rizzo CJ, Egli M, Guengerich FP, J Biol Chem. 2005 Aug 19;280(33):29750-64. Epub 2005 Jun 17. PMID:15965231
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