2fwm
Crystal Structure of E. coli EntA, a 2,3-dihydrodihydroxy benzoate dehydrogenase
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OverviewOverview
The Escherichia coli enterobactin synthetic cluster is composed of six, proteins, EntA-EntF, that form the enterobactin molecule from three serine, molecules and three molecules of 2,3-dihydroxybenzoic acid (DHB). EntC, EntB and EntA catalyze the three-step synthesis of DHB from chorismate., EntA is a member of the short-chain oxidoreductase (SCOR) family of, proteins and catalyzes the final step in DHB synthesis, the NAD+-dependent, oxidation of 2,3-dihydro-2,3-dihydroxybenzoic acid to DHB. The structure, of EntA has been determined by multi-wavelength anomalous dispersion, methods. Here, the 2.0 A crystal structure of EntA in the unliganded form, is presented. Analysis of the structure in light of recent structural and, bioinformatic analysis of other members of the SCOR family provides, insight into the residues involved in cofactor and substrate binding.
About this StructureAbout this Structure
2FWM is a Single protein structure of sequence from Escherichia coli. Active as 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase, with EC number 1.3.1.28 Full crystallographic information is available from OCA.
ReferenceReference
Determination of the crystal structure of EntA, a 2,3-dihydro-2,3-dihydroxybenzoic acid dehydrogenase from Escherichia coli., Sundlov JA, Garringer JA, Carney JM, Reger AS, Drake EJ, Duax WL, Gulick AM, Acta Crystallogr D Biol Crystallogr. 2006 Jul;62(Pt 7):734-40. Epub 2006, Jun 20. PMID:16790929
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