2ezv
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Crystal structure of tetrameric restriction endonuclease SfiI bound to cognate DNA.
OverviewOverview
Many reactions in cells proceed via the sequestration of two DNA molecules, in a synaptic complex. SfiI is a member of a growing family of restriction, enzymes that can bind and cleave two DNA sites simultaneously. We present, here the structures of tetrameric SfiI in complex with cognate DNA. The, structures reveal two different binding states of SfiI: one with both, DNA-binding sites fully occupied and the other with fully and partially, occupied sites. These two states provide details on how SfiI recognizes, and cleaves its target DNA sites, and gives insight into sequential, binding events. The SfiI recognition sequence (GGCCNNNN[downward, arrow]NGGCC) is a subset of the recognition sequence of BglI, (GCCNNNN[downward arrow]NGGC), and both enzymes cleave their target DNAs, to leave 3-base 3' overhangs. We show that even though SfiI is a tetramer, and BglI is a dimer, and there is little sequence similarity between the, two enzymes, their modes of DNA recognition are unusually similar.
About this StructureAbout this Structure
2EZV is a Single protein structure of sequence from [1] with CA as ligand. Active as Type II site-specific deoxyribonuclease, with EC number 3.1.21.4 Full crystallographic information is available from OCA.
ReferenceReference
A view of consecutive binding events from structures of tetrameric endonuclease SfiI bound to DNA., Vanamee ES, Viadiu H, Kucera R, Dorner L, Picone S, Schildkraut I, Aggarwal AK, EMBO J. 2005 Dec 7;24(23):4198-208. Epub 2005 Nov 24. PMID:16308566
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