2e1w

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Crystal structure of adenosine deaminase complexed with potent inhibitors

File:2e1w.gif


2e1w, resolution 2.5Å

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OverviewOverview

We disclose optimization efforts based on the novel non-nucleoside, adenosine deaminase (ADA) inhibitor, 4 (K(i) = 680 nM). Structure-based, drug design utilizing the crystal structure of the 4/ADA complex led to, discovery of 5 (K(i) = 11 nM, BA = 30% in rats). Furthermore, from, metabolic considerations, we discovered two inhibitors with improved oral, bioavailability [6 (K(i) = 13 nM, BA = 44%) and 7 (K(i) = 9.8 nM, BA =, 42%)]. 6 demonstrated in vivo efficacy in models of inflammation and, lymphoma.

About this StructureAbout this Structure

2E1W is a Single protein structure of sequence from Bos taurus with ZN and FR6 as ligands. This structure superseeds the now removed PDB entry 1V78. Active as Adenosine deaminase, with EC number 3.5.4.4 Full crystallographic information is available from OCA.

ReferenceReference

Structure-based design and synthesis of non-nucleoside, potent, and orally bioavailable adenosine deaminase inhibitors., Terasaka T, Okumura H, Tsuji K, Kato T, Nakanishi I, Kinoshita T, Kato Y, Kuno M, Seki N, Naoe Y, Inoue T, Tanaka K, Nakamura K, J Med Chem. 2004 May 20;47(11):2728-31. PMID:15139750

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