2dsv
Interactions of protective signalling factor with chitin-like polysaccharide: Crystal structure of the complex between signalling protein from sheep (SPS-40) and a hexasaccharide at 2.5A resolution
|
OverviewOverview
Crystal structures of four complexes of sheep secretory glycoprotein, (SPS-40) with N-acetylglucosamine oligosaccharides (GlcNAc(n), (n=3-6)), have been determined at moderate resolutions. The binding studies of, SPS-40 have been carried out using fluorescence spectroscopy and Surface, Plasmon Resonance (SPR). Structure determinations of four complexes have, shown a novel binding pattern of GlcNAc(n) molecules to SPS-40. The, results indicate that the most preferred recognition region in the, carbohydrate binding groove in SPS-40 is at subsites -4 to -2 among which, subsite -2 provides the maximum interactions with carbohydrate residues., These structures have also shown that the interactions of GlcNAc3 and, GlcNAc4 do not perturb the protein structure and those of GlcNAc5 induce, partial conformational changes while in the case of GlcNAc6 the partially, closed binding groove opened up completely. As in other SPX-40 structures, SPS-40 structure contains three overlapping flexible surface segments, His188-His197, Phe202-Arg212 and Phe244-Pro260 with several charged, residues protruding outwardly. It creates a cluster of positive charges, with a flexible base thus indicating a good scope of promoting the, intermolecular interactions. This protein is glycosylated at Asn39 and may, recognize other receptors having sugar binding sites. It appears that, SPS-40 may involve both carbohydrate and protein bindings. The systematic, carbohydrate-binding studies and the detailed structural results of four, protein-carbohydrate complexes provide an excellent insight into the, mechanism of carbohydrate binding. These are the first studies of this, kind on secretory glycoproteins and their interactions with carbohydrates.
About this StructureAbout this Structure
2DSV is a Single protein structure of sequence from Ovis aries. This structure superseeds the now removed PDB entries 2B2L and 2ANF. Full crystallographic information is available from OCA.
ReferenceReference
Carbohydrate binding properties and carbohydrate induced conformational switch in sheep secretory glycoprotein (SPS-40): crystal structures of four complexes of SPS-40 with chitin-like oligosaccharides., Srivastava DB, Ethayathulla AS, Kumar J, Somvanshi RK, Sharma S, Dey S, Singh TP, J Struct Biol. 2007 Jun;158(3):255-66. Epub 2006 Nov 17. PMID:17188513
Page seeded by OCA on Wed Nov 21 09:45:51 2007