2d6b
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Novel Bromate Species trapped within a Protein Crystal
OverviewOverview
Only a few protein-oxoanion crystal complexes have been described to date., Here, the structure of a protein soaked in a bromate solution has been, determined to a resolution of 1.25 A and refined to final overall, R/R(free) values of 18.04/21.3 (isotropic) and 11.25/14.67 (anisotropic)., In contrast to the single-model approach, refinement of an ensemble of ten, models enabled us to determine variances and statistically evaluate, bond-length distances and angles in the oxoanions. In total, nine bromate, positions, including two BrO(3)(-) x HBrO(3) dimer species, have been, identified on the basis of the anomalous signal of the Br atoms. For all, bromate ions, the main-chain amide atoms of the protein were identified as, the dominant binding positions, a useful property in any experimental, phase-determination experiment.
About this StructureAbout this Structure
2D6B is a Single protein structure of sequence from Gallus gallus with CL, NA and 202 as ligands. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.
ReferenceReference
An ensemble of crystallographic models enables the description of novel bromate-oxoanion species trapped within a protein crystal., Ondracek J, Mesters JR, Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):996-1001. Epub 2006, Aug 19. PMID:16929100
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