2bu1
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MS2-RNA HAIRPIN (5BRU-5) COMPLEX
OverviewOverview
We have determined the X-ray structures of six MS2 RNA, hairpin-coat-protein complexes having five different substitutions at the, hairpin loop base -5. This is a uracil in the wild-type hairpin and, contacts the coat protein both by stacking on to a tyrosine side chain and, by hydrogen bonding to an asparagine side chain. The RNA consensus, sequence derived from coat protein binding studies with natural sequence, variants suggested that the -5 base needs to be a pyrimidine for strong, binding. The five -5 substituents used in this study were 5-bromouracil, pyrimidin-2-one, 2-thiouracil, adenine, and guanine. The structure of the, 5-bromouracil complex was determined to 2.2 A resolution, which is the, highest to date for any MS2 RNA-protein complex. All the complexes, presented here show very similar conformations, despite variation in, affinity in solution. The results suggest that the stacking of the -5 base, on to the tyrosine side chain is the most important driving force for, complex formation. A number of hydrogen bonds that are present in the, wild-type complex are not crucial for binding, as they are missing in one, or more of the complexes. The results also reveal the flexibility of this, RNA-protein interface, with respect to functional group variation, and may, be generally applicable to other RNA-protein complexes.
About this StructureAbout this Structure
2BU1 is a Protein complex structure of sequences from Enterobacterio phage ms2. This structure superseeds the now removed PDB entry 1E6T. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis of pyrimidine specificity in the MS2 RNA hairpin-coat-protein complex., Grahn E, Moss T, Helgstrand C, Fridborg K, Sundaram M, Tars K, Lago H, Stonehouse NJ, Davis DR, Stockley PG, Liljas L, RNA. 2001 Nov;7(11):1616-27. PMID:11720290
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- Enterobacterio phage ms2
- Protein complex
- Davis, D.
- Fridborg, K.
- Grahn, E.
- Helgstrand, C.
- Lago, H.
- Liljas, L.
- Moss, T.
- Stockley, P.
- Stonehouse, N.
- Sundaram, M.
- Tars, K.
- Capsid
- Capsid protein
- Complex (capsid protein/rna hairpin)
- Hairpin
- Icosahedral virus
- Levivirus
- Rna-binding
- Structural protein