2b10
Crystal Structure of the Protein-Protein Complex between F82S cytochrome c and cytochrome c peroxidase
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OverviewOverview
Although bonding networks determine electron-transfer (ET) rates within, proteins, the mechanism by which structure and dynamics influence ET, across protein interfaces is not well understood. Measurements of, photochemically induced ET and subsequent charge recombination between, Zn-porphyrin-substituted cytochrome c peroxidase and cytochrome c in, single crystals correlate reactivity with defined structures for different, association modes of the redox partners. Structures and ET rates in, crystals are consistent with tryptophan oxidation mediating charge, recombination reactions. Conservative mutations at the interface can, drastically affect how the proteins orient and dispose redox centers., Whereas some configurations are ET inactive, the wild-type complex, exhibits the fastest recombination rate. Other association modes generate, ET rates that do not correlate with predictions based on cofactor, separations or simple bonding pathways. Inhibition of photoinduced ET at, <273 K indicates gating by small-amplitude dynamics, even within the, crystal. Thus, different associations achieve states of similar, reactivity, and within those states conformational fluctuations enable, interprotein ET.
About this StructureAbout this Structure
2B10 is a Protein complex structure of sequences from Saccharomyces cerevisiae with ZNH and HEM as ligands. Active as Cytochrome-c peroxidase, with EC number 1.11.1.5 Full crystallographic information is available from OCA.
ReferenceReference
Effects of interface mutations on association modes and electron-transfer rates between proteins., Kang SA, Crane BR, Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15465-70. Epub 2005 Oct 14. PMID:16227441
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