2adz
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solution structure of the joined PH domain of alpha1-syntrophin
OverviewOverview
Pleckstrin homology (PH) domains play diverse roles in cytoskeletal, dynamics and signal transduction. Split PH domains represent a unique, subclass of PH domains that have been implicated in interactions with, complementary partial PH domains 'hidden' in many proteins. Whether, partial PH domains exist as independent structural units alone and whether, two halves of a split PH domain can fold together to form an intact PH, domain are not known. Here, we solved the structure of the PH(N)-PDZ-PH(C), tandem of alpha-syntrophin. The split PH domain of alpha-syntrophin adopts, a canonical PH domain fold. The isolated partial PH domains of, alpha-syntrophin, although completely unfolded, remain soluble in, solution. Mixing of the two isolated domains induces de novo folding and, yields a stable PH domain. Our results demonstrate that two complementary, partial PH domains are capable of binding to each other to form an intact, PH domain. We further showed that the PH(N)-PDZ-PH(C) tandem forms a, functionally distinct supramodule, in which the split PH domain and the, PDZ domain function synergistically in binding to inositol phospholipids.
About this StructureAbout this Structure
2ADZ is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
Structure of the split PH domain and distinct lipid-binding properties of the PH-PDZ supramodule of alpha-syntrophin., Yan J, Wen W, Xu W, Long JF, Adams ME, Froehner SC, Zhang M, EMBO J. 2005 Dec 7;24(23):3985-95. Epub 2005 Oct 27. PMID:16252003
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