1zg5
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NarL complexed to narG-89 promoter palindromic tail-to-tail DNA site
OverviewOverview
NarL is a model response regulator for bacterial two-component signal, transduction. The NarL C-terminal domain DNA binding domain alone, (NarL(C)) contains all essential DNA binding determinants of the, full-length NarL transcription factor. In the full-length NarL protein, the N-terminal regulatory domain must be phosphorylated to release the DNA, binding determinants; however, the first NarL(C)-DNA cocrystal structure, showed that dimerization of NarL(C) on DNA occurs in a manner independent, of the regulatory domain [Maris, A. E., et al. (2002) Nat. Struct. Biol., 9, 771-778]. Dimerization via the NarL(C) C-terminal helix conferred, high-affinity recognition of the tail-to-tail promoter site arrangement., Here, two new cocrystal structures are presented of NarL(C) complexed with, additional 20mer oligonucleotides representative of other high-affinity, tail-to-tail NarL binding sites found in upstream promoter regions. DNA, structural recognition properties are described, such as backbone, flexibility and groove width, that facilitate NarL(C) dimerization and, high-affinity recognition. Lys 188 on the recognition helix accommodates, DNA sequence variation between the three different cocomplexes by, providing flexible specificity, recognizing the DNA major groove floor, directly and/or via bridging waters. The highly conserved Val 189, which, enforced significant DNA base distortion in the first cocrystal structure, enforces similar distortions in the two new cocrystal structures., Recognition also is conserved for Lys 192, which hydrogen bonds to, guanines at regions of high DNA helical writhe. DNA affinity measurements, for model NarL binding sites, including those that did not cocrystallize, suggest a framework for explaining the diversity of heptamer site, arrangement and orientation.
About this StructureAbout this Structure
1ZG5 is a Single protein structure of sequence from Escherichia coli with SO4 as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Primary and secondary modes of DNA recognition by the NarL two-component response regulator., Maris AE, Kaczor-Grzeskowiak M, Ma Z, Kopka ML, Gunsalus RP, Dickerson RE, Biochemistry. 2005 Nov 8;44(44):14538-52. PMID:16262254
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