1zba
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Foot-and-Mouth Disease virus serotype A1061 complexed with oligosaccharide receptor.
OverviewOverview
Foot-and-mouth disease viruses (FMDVs) target epithelial cells via, integrin receptors, but can acquire the capacity to bind cell-surface, heparan sulphate (or alternative receptors) on passage in cell culture., Vaccine viruses must be propagated in cell culture and, hence, some, rationale for the selection of variants in this process is important., Crystal structures are available for type O, A and C viruses and also for, a complex of type O strain O(1)BFS with heparin. The structure of FMDV, A10(61) (a cell culture-adapted strain) complexed with heparin has now, been determined. This virus has an RGSD motif in place of the otherwise, conserved RGD integrin-binding motif and the potential to bind heparan, sulphate (suggested by sequence analyses). FMDV A10(61) was closely, similar in structure to other serotypes, deviating most in antigenic, sites. The VP1 GH loop comprising the integrin-binding motif was, disordered. Heparin bound at a similar site and in a similar conformation, to that seen in the analogous complex with O(1)BFS, although the binding, had a lower affinity and was more ionic.
About this StructureAbout this Structure
1ZBA is a Protein complex structure of sequences from Foot-and-mouth disease virus. Full crystallographic information is available from OCA.
ReferenceReference
Structure of Foot-and-mouth disease virus serotype A10 61 alone and complexed with oligosaccharide receptor: receptor conservation in the face of antigenic variation., Fry EE, Newman JW, Curry S, Najjam S, Jackson T, Blakemore W, Lea SM, Miller L, Burman A, King AM, Stuart DI, J Gen Virol. 2005 Jul;86(Pt 7):1909-20. PMID:15958669
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