1yvr

OverviewOverview

The Ro 60 kDa autoantigen is a major target of the immune response in, patients with systemic lupus erythematosus. In vertebrate cells, Ro binds, misfolded small RNAs and likely functions in RNA quality control. In, eukaryotes and bacteria, Ro also associates with small RNAs called Y RNAs., We present structures of unliganded Ro and Ro complexed with two RNAs at, 1.95 and 2.2 A resolution, respectively. Ro consists of a von Willebrand, factor A domain and a doughnut-shaped domain composed of HEAT repeats. In, the complex, a fragment of Y RNA binds on the outer surface of the, HEAT-repeat ring, and single-stranded RNA binds in the toroid hole., Mutagenesis supports a binding site for misfolded RNAs that encompasses, both sites, with a single-stranded end inserted into the toroid cavity., Our experiments suggest that one role of Y RNAs may be to regulate access, of other RNAs to Ro.

About this StructureAbout this Structure

1YVR is a Single protein structure of sequence from Xenopus laevis. Full crystallographic information is available from OCA.

ReferenceReference

Structural insights into RNA quality control: the Ro autoantigen binds misfolded RNAs via its central cavity., Stein AJ, Fuchs G, Fu C, Wolin SL, Reinisch KM, Cell. 2005 May 20;121(4):529-39. PMID:15907467

Page seeded by OCA on Wed Nov 21 07:05:14 2007

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA