2bkv

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STRUCTURE AND KINETICS OF A MONOMERIC GLUCOSAMINE-6-PHOSPHATE DEAMINASE: MISSING LINK OF THE NAGB SUPERFAMILY

File:2bkv.gif


2bkv, resolution 1.504Å

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OverviewOverview

Glucosamine 6-phosphate is converted to fructose 6-phosphate and ammonia, by the action of the enzyme glucosamine 6-phosphate deaminase, NagB. This, reaction is the final step in the specific GlcNAc utilization pathway and, thus decides the metabolic fate of GlcNAc. Sequence analyses suggest that, the NagB "superfamily" consists of three main clusters: multimeric and, allosterically regulated glucosamine-6-phosphate deaminases (exemplified, by Escherichia coli NagB), phosphogluconolactonases, and monomeric, hexosamine-6-phosphate deaminases. Here we present the three-dimensional, structure and kinetics of the first member of this latter group, the, glucosamine-6-phosphate deaminase, NagB, from Bacillus subtilis. The, structures were determined in ligand-complexed forms at resolutions ... [(full description)]

About this StructureAbout this Structure

2BKV is a [Single protein] structure of sequence from [Bacillus subtilis] with PGA as [ligand]. Active as [[1]], with EC number [3.5.99.6]. Full crystallographic information is available from [OCA].

ReferenceReference

Structure and kinetics of a monomeric glucosamine 6-phosphate deaminase: missing link of the NagB superfamily?, Vincent F, Davies GJ, Brannigan JA, J Biol Chem. 2005 May 20;280(20):19649-55. Epub 2005 Mar 8. PMID:15755726

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