1yfe
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Crystal structure of apo fumarase C from Escherichia coli
OverviewOverview
Previous crystal structures of fumarase C from Escherichia coli have noted, two occupied dicarboxylate-binding sites termed the active site and the B, site. Here, the first known fumarase C structure is reported in which both, sites are unoccupied by bound ligand. This so-called ;free' crystal form, shows conservation of the active-site water in a similar orientation to, that reported in other fumarase C crystal structures. More importantly, a, shift of His129 has been observed at the B site. This new crystallographic, information suggests the use of water as a permanent member of the active, site and the use of an imidazole-imidazolium conversion to control access, at the allosteric B site.
About this StructureAbout this Structure
1YFE is a Single protein structure of sequence from Escherichia coli. Active as Fumarate hydratase, with EC number 4.2.1.2 Full crystallographic information is available from OCA.
ReferenceReference
Structure of free fumarase C from Escherichia coli., Weaver T, Acta Crystallogr D Biol Crystallogr. 2005 Oct;61(Pt 10):1395-401. Epub, 2005 Sep 28. PMID:16204892
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