1o7y

Revision as of 22:26, 29 October 2007 by OCA (talk | contribs) (New page: left|200px<br /> <applet load="1o7y" size="450" color="white" frame="true" align="right" spinBox="true" caption="1o7y, resolution 3.00Å" /> '''CRYSTAL STRUCTURE O...)
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CRYSTAL STRUCTURE OF IP-10 M-FORM

File:1o7y.gif


1o7y, resolution 3.00Å

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OverviewOverview

We have determined the structure of wild-type IP-10 from three crystal, forms. The crystals provide eight separate models of the IP-10 chain, all, differing substantially from a monomeric IP-10 variant examined previously, by NMR spectroscopy. In each crystal form, IP-10 chains form conventional, beta sheet dimers, which, in turn, form a distinct tetrameric assembly., The M form tetramer is reminiscent of platelet factor 4, whereas the T and, H forms feature a novel twelve-stranded beta sheet. Analytical, ultracentrifugation indicates that, in free solution, IP-10 exists in a, monomer-dimer equilibrium with a dissociation constant of 9 microM. We, propose that the tetrameric structures may represent species promoted by, the binding of glycosaminoglycans. The binding sites for several, ... [(full description)]

About this StructureAbout this Structure

1O7Y is a [Single protein] structure of sequence from [[1]] with SO4 as [ligand]. Full crystallographic information is available from [OCA].

ReferenceReference

Crystal structures of oligomeric forms of the IP-10/CXCL10 chemokine., Swaminathan GJ, Holloway DE, Colvin RA, Campanella GK, Papageorgiou AC, Luster AD, Acharya KR, Structure. 2003 May;11(5):521-32. PMID:12737818

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