1wac
BACK-PRIMING MODE OF PHI6 RNA-DEPENDENT RNA POLYMERASE
|
OverviewOverview
The RNA-dependent RNA polymerase of the double-stranded RNA bacteriophage, phi6 is capable of primer-independent initiation, as are many RNA, polymerases. The structure of this polymerase revealed an initiation, platform, composed of a loop in the C-terminal domain (QYKW, aa 629-632), that was essential for de novo initiation. A similar element has been, identified in hepatitis C virus RNA-dependent RNA polymerase. Biochemical, studies have addressed the role of this platform, revealing that a mutant, version can utilize a back-priming initiation mechanism, where the 3', terminus of the template adopts a hairpin-like conformation. Here, the, mechanism of back-primed initiation is studied further by biochemical and, structural methods.
About this StructureAbout this Structure
1WAC is a Single protein structure of sequence from Bacteriophage phi-6. Full crystallographic information is available from OCA.
ReferenceReference
Back-priming mode of phi6 RNA-dependent RNA polymerase., Laurila MR, Salgado PS, Stuart DI, Grimes JM, Bamford DH, J Gen Virol. 2005 Feb;86(Pt 2):521-6. PMID:15659773
Page seeded by OCA on Wed Nov 21 05:16:39 2007