1wae

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Revision as of 22:12, 29 October 2007 by OCA (talk | contribs) (New page: left|200px<br /> <applet load="1wae" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wae, resolution 1.95Å" /> '''CRYSTAL STRUCTURE O...)
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File:1wae.gif


1wae, resolution 1.95Å

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CRYSTAL STRUCTURE OF H129V MUTANT OF ALCALIGENES XYLOSOXIDANS NITRITE REDUCTASE

OverviewOverview

Copper nitrite reductases contain both an electron-transfer type 1 Cu site, and a catalytic type 2 Cu site. We have mutated one of the type 2 copper, ligating histidines to observe the effect on catalytic turnover. This, mutation has created a unique site where Cu is ligated by 2 His Nepsilon2, atoms alone.

About this StructureAbout this Structure

1WAE is a [Single protein] structure of sequence from [Alcaligenes xylosoxydans xylosoxydans] with CU and ZN as [ligands]. Active as [[1]], with EC number [1.7.99.3]. Full crystallographic information is available from [OCA].

ReferenceReference

Observation of an unprecedented Cu Bis-His site: crystal structure of the H129V mutant of nitrite reductase., Ellis MJ, Antonyuk SV, Strange RW, Sawers G, Eady RR, Hasnain SS, Inorg Chem. 2004 Nov 29;43(24):7591-3. PMID:15554622

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