1u1b
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Structure of bovine pancreatic Ribonuclease A in complex with 3'-phosphothymidine (3'-5')-pyrophosphate adenosine 3'-phosphate
OverviewOverview
Solution NMR spin-relaxation experiments were used to compare mus-ms, dynamics in RNase A in the apo form and as complexed to the, substrate-mimic, pTppAp. The crystal structure of the RNase A/pTppAp, complex was determined and demonstrates that this ligand binds at the, active site and utilizes established substrate binding sites in its, interaction with RNase A. Relaxation-compensated CPMG experiments identify, flexible residues in and around the active site in both the apo and, pTppAp-bound enzyme. Quantitative analysis of the NMR spin-relaxation, dispersion curves show that the time scale of motion in RNase A is, unchanged when pTppAp binds and is similar to the time scale for the, rate-determining step of the catalytic reaction. Temperature-dependent, measurements provide an activation barrier for motion of 5.2 +/- 1.0, kcal/mol and 4.5 +/- 1.2 kcal/mol for the apo and pTppAp forms of RNase A, respectively. These data indicate very similar motion exists in the free, and bound enzyme. Additionally, chemical shift data suggests that the, magnitude of motion is also similar for these two forms and that it is, likely that apo enzyme interconverts to a structure that resembles a, ligand-bound form. Likewise, it appears that the bound conformation, samples the apo enzyme form even when ligand is present. Taken together, the data imply that RNase A is in a preexisting dynamic equilibrium, between two conformations that represent the open and closed enzyme forms., These data suggest that ligand binding stabilizes the bound conformer but, does not induce it.
About this StructureAbout this Structure
1U1B is a Single protein structure of sequence from Bos taurus with PAX as ligand. Active as Pancreatic ribonuclease, with EC number 3.1.27.5 Full crystallographic information is available from OCA.
ReferenceReference
Conservation of mus-ms enzyme motions in the apo- and substrate-mimicked state., Beach H, Cole R, Gill ML, Loria JP, J Am Chem Soc. 2005 Jun 29;127(25):9167-76. PMID:15969595
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