1tzd

Revision as of 04:36, 21 November 2007 by OCA (talk | contribs) (New page: left|200px<br /><applet load="1tzd" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tzd, resolution 2.20Å" /> '''CRYSTAL STRUCTURE OF...)
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CRYSTAL STRUCTURE OF THE CATALYTIC CORE OF INOSITOL 1,4,5-TRISPHOSPHATE 3-KINASE

File:1tzd.jpg


1tzd, resolution 2.20Å

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OverviewOverview

Soluble inositol polyphosphates are ubiquitous second messengers in, eukaryotes, and their levels are regulated by an array of specialized, kinases. The structure of an archetypal member of this class, inositol, 1,4,5-trisphosphate 3-kinase (IP3K), has been determined at 2.2 angstroms, resolution in complex with magnesium and adenosine diphosphate. IP3K, contains a catalytic domain that is a variant of the protein kinase, superfamily, and a novel four-helix substrate binding domain. The two, domains are in an open conformation with respect to each other, suggesting, that substrate recognition and catalysis by IP3K involves a dynamic, conformational cycle. The unique helical domain of IP3K blocks access to, the active site by membrane-bound phosphoinositides, explaining the, structural basis for soluble inositol polyphosphate specificity.

About this StructureAbout this Structure

1TZD is a Single protein structure of sequence from Rattus norvegicus with MG and ADP as ligands. Active as Inositol-trisphosphate 3-kinase, with EC number 2.7.1.127 Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the catalytic core of inositol 1,4,5-trisphosphate 3-kinase., Miller GJ, Hurley JH, Mol Cell. 2004 Sep 10;15(5):703-11. PMID:15350215

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