1tri
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THE CRYSTAL STRUCTURE OF AN ENGINEERED MONOMERIC TRIOSEPHOSPHATE ISOMERASE, MONOTIM: THE CORRECT MODELLING OF AN EIGHT-RESIDUE LOOP
OverviewOverview
BACKGROUND: The triosephosphate isomerase (TIM) fold is found in several, different classes of enzymes, most of which are oligomers; TIM itself, always functions as a very tight dimer. It has recently been shown that a, monomeric form of TIM ('monoTIM') can be constructed by replacing a, 15-residue interface loop, loop-3, with an eight-residue fragment;, modelling suggests that this should result in a short strain-free turn, resulting in the subsequent helix, helix-A3, having an additional turn at, its amino terminus. RESULTS: The crystal structure of monoTIM shows that, it retains the characteristic TIM-barrel (betaalpha)8-fold and that the, new loop has a structure very close to that predicted. Two other interface, loops, loop-1 and loop-4, which contain the active site residues Lys13 and, His95, respectively, show significant changes in structure in monoTIM, compared with dimeric wild-type TIM. CONCLUSION: The observed structural, differences between monoTIM and wild-type TIM indicate that the dimeric, appearance of TIM determines the location and conformation of two of the, four catalytic residues.
About this StructureAbout this Structure
1TRI is a Single protein structure of sequence from Trypanosoma brucei brucei with SO4 as ligand. Active as Triose-phosphate isomerase, with EC number 5.3.1.1 Full crystallographic information is available from OCA.
ReferenceReference
The crystal structure of an engineered monomeric triosephosphate isomerase, monoTIM: the correct modelling of an eight-residue loop., Borchert TV, Abagyan R, Kishan KV, Zeelen JP, Wierenga RK, Structure. 1993 Nov 15;1(3):205-13. PMID:16100954
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