1t7f
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Crystal structure of the androgen receptor ligand binding domain in complex with a LxxLL motif
OverviewOverview
Prostate cancer is a leading killer of men in the industrialized world., Underlying this disease is the aberrant action of the androgen receptor, (AR). AR is distinguished from other nuclear receptors in that after, hormone binding, it preferentially responds to a specialized set of, coactivators bearing aromatic-rich motifs, while responding poorly to, coactivators bearing the leucine-rich "NR box" motifs favored by other, nuclear receptors. Under normal conditions, interactions with these, AR-specific coactivators through aromatic-rich motifs underlie targeted, gene transcription. However, during prostate cancer, abnormal association, with such coactivators, as well as with coactivators containing canonical, leucine-rich motifs, promotes disease progression. To understand the, paradox of this unusual selectivity, we have derived a complete set of, peptide motifs that interact with AR using phage display. Binding, affinities were measured for a selected set of these peptides and their, interactions with AR determined by X-ray crystallography. Structures of AR, in complex with FxxLF, LxxLL, FxxLW, WxxLF, WxxVW, FxxFF, and FxxYF motifs, reveal a changing surface of the AR coactivator binding interface that, permits accommodation of both AR-specific aromatic-rich motifs and, canonical leucine-rich motifs. Induced fit provides perfect mating of the, motifs representing the known family of AR coactivators and suggests a, framework for the design of AR coactivator antagonists.
About this StructureAbout this Structure
1T7F is a Single protein structure of sequence from Pan troglodytes with DHT as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Recognition and accommodation at the androgen receptor coactivator binding interface., Hur E, Pfaff SJ, Payne ES, Gron H, Buehrer BM, Fletterick RJ, PLoS Biol. 2004 Sep;2(9):E274. Epub 2004 Aug 24. PMID:15328534
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