1sdj
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X-RAY STRUCTURE OF YDDE_ECOLI NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET ET25.
OverviewOverview
Phenazines produced by Pseudomonas and Streptomyces spp. are heterocyclic, nitrogen-containing metabolites with antibiotic, antitumor, and, antiparasitic activity. The antibiotic properties of pyocyanin, produced, by Pseudomonas aeruginosa, were recognized in the 1890s, although this, blue phenazine is now known to be a virulence factor in human disease., Despite their biological significance, the biosynthesis of phenazines is, not fully understood. Here we present structural and functional studies of, PhzF, an enzyme essential for phenazine synthesis in Pseudomonas spp. PhzF, shares topology with diaminopimelate epimerase DapF but lacks the same, catalytic residues. The structure of PhzF in complex with its substrate, trans-2,3-dihydro-3-hydroxyanthranilic acid, suggests that it is an, isomerase using the conserved glutamate E45 to abstract a proton from C3, of the substrate. The proton is returned to C1 of the substrate after, rearrangement of the double-bond system, yielding an enol that converts to, the corresponding ketone. PhzF is a dimer that may be bifunctional, providing a shielded cavity for ketone dimerization via double Schiff-base, formation to produce the phenazine scaffold. Our proposed mechanism is, supported by mass and NMR spectroscopy. The results are discussed in the, context of related structures and protein sequences of unknown biochemical, function.
About this StructureAbout this Structure
1SDJ is a Single protein structure of sequence from Escherichia coli with SO4 as ligand. This structure superseeds the now removed PDB entry 1P9V. Full crystallographic information is available from OCA.
ReferenceReference
Structure and function of the phenazine biosynthetic protein PhzF from Pseudomonas fluorescens., Blankenfeldt W, Kuzin AP, Skarina T, Korniyenko Y, Tong L, Bayer P, Janning P, Thomashow LS, Mavrodi DV, Proc Natl Acad Sci U S A. 2004 Nov 23;101(47):16431-6. Epub 2004 Nov 15. PMID:15545603
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