1rbj

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Revision as of 02:19, 21 November 2007 by OCA (talk | contribs) (New page: left|200px<br /><applet load="1rbj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rbj, resolution 2.700Å" /> '''RIBONUCLEASE B COMP...)
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File:1rbj.gif


1rbj, resolution 2.700Å

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RIBONUCLEASE B COMPLEX WITH D(TETRA-(DEOXY-ADENYLATE))

OverviewOverview

The structure of a tetragonal crystal of bovine pancreatic RNase B, complexed with d(pA)(4) was determined by molecular replacement and, difference Fourier methods. This crystal belongs to space group P4(1)2(1)2, and has unit-cell dimensions a = b = 44.5, c = 156.5 A. The model consists, of the enzyme and a tetranucleotide with fractional occupancies, suggesting multiple modes of oligonucleotide binding. It does not include, any polysaccharide residues or solvent molecules. After refinement at 2.7, A, the R value was 0.163 with acceptable stereochemistry. The model, illustrates a set of well defined interactions for substrate binding, particularly between the central dinucleotide and the enzyme.

About this StructureAbout this Structure

1RBJ is a Single protein structure of sequence from Bos taurus. Active as Pancreatic ribonuclease, with EC number 3.1.27.5 Full crystallographic information is available from OCA.

ReferenceReference

Structure of a ribonuclease B+d(pA)4 complex., Ko TP, Williams R, McPherson A, Acta Crystallogr D Biol Crystallogr. 1996 Jan 1;52(Pt 1):160-4. PMID:15299737

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