1r0h

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Revision as of 02:02, 21 November 2007 by OCA (talk | contribs) (New page: left|200px<br /><applet load="1r0h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r0h, resolution 1.70Å" /> '''cobalt-substituted r...)
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File:1r0h.jpg


1r0h, resolution 1.70Å

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cobalt-substituted rubredoxin

OverviewOverview

Five different metal-substituted forms of Clostridium pasteurianum, rubredoxin have been prepared and crystallized. The single Fe atom present, in the Fe(S-Cys)(4) site of the native form of the protein was exchanged, in turn for Co, Ni, Ga, Cd and Hg. All five forms of rubredoxin, crystallized in space group R3 and were isomorphous with the native, protein. The Co-, Ni- and Ga-substituted proteins exhibited metal sites, with geometries similar to that of the Fe form (effective D(2d) local, symmetry), as did the Cd and Hg proteins, but with a significant expansion, of the metal-sulfur bond lengths. A knowledge of these structures, contributes to a molecular understanding of the function of this simple, iron-sulfur electron-transport protein.

About this StructureAbout this Structure

1R0H is a Single protein structure of sequence from Clostridium pasteurianum with CO as ligand. Full crystallographic information is available from OCA.

ReferenceReference

Metal-substituted derivatives of the rubredoxin from Clostridium pasteurianum., Maher M, Cross M, Wilce MC, Guss JM, Wedd AG, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):298-303. Epub 2004, Jan 23. PMID:14747706

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