1pd2
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CRYSTAL STRUCTURE OF HEMATOPOIETIC PROSTAGLANDIN D SYNTHASE COMPLEX WITH GLUTATHIONE
OverviewOverview
Hematopoietic prostaglandin (PG) D synthase is the key enzyme for, production of the D and J series of prostanoids in the immune system and, mast cells. We isolated a cDNA for the rat enzyme, crystallized the, recombinant enzyme, and determined the three-dimensional structure of the, enzyme complexed with glutathione at 2.3 A resolution. The enzyme is the, first member of the sigma class glutathione S-transferase (GST) from, vertebrates and possesses a prominent cleft as the active site, which is, never seen among other members of the GST family. The unique 3-D, architecture of the cleft leads to the putative substrate binding mode and, its catalytic mechanism, responsible for the specific isomerization from, PGH2 to PGD2.
About this StructureAbout this Structure
1PD2 is a Single protein structure of sequence from Rattus norvegicus with GTT as ligand. Active as Prostaglandin-D synthase, with EC number 5.3.99.2 Full crystallographic information is available from OCA.
ReferenceReference
Cloning and crystal structure of hematopoietic prostaglandin D synthase., Kanaoka Y, Ago H, Inagaki E, Nanayama T, Miyano M, Kikuno R, Fujii Y, Eguchi N, Toh H, Urade Y, Hayaishi O, Cell. 1997 Sep 19;90(6):1085-95. PMID:9323136
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