1oy3
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CRYSTAL STRUCTURE OF AN IKBBETA/NF-KB P65 HOMODIMER COMPLEX
OverviewOverview
We report the crystal structure of a murine IkappaBbeta x NF-kappaB p65, homodimer complex. Crystallographic models were determined for two, triclinic crystalline systems and refined against data at 2.5 and 2.1 A., The overall complex structure is similar to that of the, IkappaBalpha.NF-kappaB p50/p65 heterodimer complex. One NF-kappaB p65, subunit nuclear localization signal clearly contacts IkappaBbeta, whereas, a homologous segment from the second subunit of the homodimer is mostly, solvent-exposed. The unique 47-amino acid insertion between ankyrin, repeats three and four of IkappaBbeta is mostly disordered in the, structure. Primary sequence analysis and differences in the mode of, binding at the IkappaBbeta sixth ankyrin repeat and NF-kappaB p65, homodimer suggest a model for nuclear IkappaBbeta.NF-kappaB.DNA ternary, complex formation. These unique structural features of IkappaBbeta may, contribute to its ability to mediate persistent NF-kappaB activation.
About this StructureAbout this Structure
1OY3 is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
X-ray crystal structure of an IkappaBbeta x NF-kappaB p65 homodimer complex., Malek S, Huang DB, Huxford T, Ghosh S, Ghosh G, J Biol Chem. 2003 Jun 20;278(25):23094-100. Epub 2003 Apr 9. PMID:12686541
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