1oqs
Crystal Structure of RV4/RV7 Complex
|
OverviewOverview
The presynaptic viperotoxin F is the major lethal component of the venom, of Vipera russelli formosensis (Taiwan viper). It is a heterodimer of two, highly homologous (65% identity) but oppositely charged subunits: a basic, and neurotoxic PLA(2) (RV-4) and an acidic non-toxic component with a very, low enzymatic activity (RV-7). The crystal structure of the complex has, been determined by molecular replacement and refined to 1.9 A resolution, and an R factor of 22.3% with four RV-4/RV-7 complexes in the asymmetric, unit, which do not exhibit any local point-group symmetry. The complex, formation decreases the accessible surface area of the two subunits by, approximately 1425 A(2). Both PLA(2)s are predicted to have very low, if, any, anticoagulant activity. The structure of viperotoxin F is compared, with that of the heterodimeric neurotoxin vipoxin from the venom of, another viper, V. ammodytes meridionalis. The structural basis for the, differences between the pharmacological activities of the two toxins is, discussed. The neutralization of the negative charge of the major ligand, for Ca(2+), Asp49, by intersubunit salt bridges is probably a common, mechanism of self-stabilization of heterodimeric Viperinae snake-venom, neurotoxins in the absence of bound calcium.
About this StructureAbout this Structure
1OQS is a Protein complex structure of sequences from Daboia russellii siamensis. Active as Phospholipase A(2), with EC number 3.1.1.4 Full crystallographic information is available from OCA.
ReferenceReference
Structure of the heterodimeric neurotoxic complex viperotoxin F (RV-4/RV-7) from the venom of Vipera russelli formosensis at 1.9 A resolution., Perbandt M, Tsai IH, Fuchs A, Banumathi S, Rajashankar KR, Georgieva D, Kalkura N, Singh TP, Genov N, Betzel C, Acta Crystallogr D Biol Crystallogr. 2003 Oct;59(Pt 10):1679-87. Epub 2003, Sep 19. PMID:14501106
Page seeded by OCA on Tue Nov 20 23:06:50 2007