1nli
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Complex of [E160A-E189A] trichosanthin and adenine
OverviewOverview
Trichosanthin is a ribosome-inactivating protein that cleaves specifically, the N-glycosidic bond of A-4324 of 28S rRNA. Trichosanthin and its variant, [E160A-E189A]-trichosanthin were found to bind an adenine base with a K(d), value of approximately 0.2mM. To determine how this doubly mutated variant, of trichosanthin interacts with adenine, the co-crystal structure of, [E160A-E189A]-trichosanthin and adenine was resolved to 0.193nm which, revealed that the active site conformation of the doubly mutated variant, is isomorphous to wild-type trichosanthin. Water molecules were found at, locations corresponding to the eliminated side chain of Glu-160 and, Glu-189. On the other hand, the adenine base interacted with, [E160A-E189A]-trichosanthin in a manner similar to that in wild-type, trichosanthin. Our structural analysis illustrates that Glu-160 and, Glu-189 in trichosanthin do not play an important role in maintaining the, active site conformation and binding adenine, an essential step for, substrate-enzyme interaction. On the other hand, removal of two glutamate, residues changed a large patch of negatively charged surface to a positive, charge, which may account for the destabilization of the oxocarbenium-like, transition-state and the significant decrease in ribosome-inactivating, activity in [E160A-E189A]-trichosanthin.
About this StructureAbout this Structure
1NLI is a Single protein structure of sequence from Trichosanthes kirilowii with ADE as ligand. Active as rRNA N-glycosylase, with EC number 3.2.2.22 Full crystallographic information is available from OCA.
ReferenceReference
Structural basis for the interaction of [E160A-E189A]-trichosanthin with adenine., Shaw PC, Wong KB, Chan DS, Williams RL, Toxicon. 2003 Apr;41(5):575-81. PMID:12676436
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