1n2d
|
Ternary complex of MLC1P bound to IQ2 and IQ3 of Myo2p, a class V myosin
OverviewOverview
Mlc1p is a calmodulin-like protein from the budding yeast Saccharomyces, cerevisiae, where it has been identified as a subunit of a class V myosin, Myo2p, and a binding partner of an IQGAP-like protein, Iqg1p. Through its, interactions with these two proteins, Mlc1p plays a role in polarized, growth and cytokinesis. Mlc1p has been crystallized in complexes with four, different IQ target motifs from the neck region of Myo2p: IQ2, IQ3, IQ4, and IQ2-IQ3 (referred to as IQ2,3). Electron-density maps for two of the, complexes (Mlc1p-IQ4 and Mlc1p-IQ2,3) were obtained from multiple, anomalous dispersion (MAD) experiments based on selenomethionine, derivatives. The other two structures (Mlc1p-IQ2 and Mlc1p-IQ3) were, determined by molecular replacement using the partially refined structure, of Mlc1p-IQ2,3 as a search model.
About this StructureAbout this Structure
1N2D is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
Crystallization, X-ray characterization and selenomethionine phasing of Mlc1p bound to IQ motifs from myosin V., Terrak M, Otterbein LR, Wu G, Palecanda LA, Lu RC, Dominguez R, Acta Crystallogr D Biol Crystallogr. 2002 Oct;58(Pt 10 Pt 2):1882-5. Epub, 2002 Sep 28. PMID:12351846
Page seeded by OCA on Tue Nov 20 21:53:25 2007