1mok
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NADPH DEPENDENT 2-KETOPROPYL COENZYME M OXIDOREDUCTASE/CARBOXYLASE
OverviewOverview
The NADPH:2-ketopropyl-coenzyme M oxidoreductase/carboxylase (2-KPCC) is, the terminal enzyme in a metabolic pathway that results in the conversion, of propylene to the central metabolite acetoacetate in Xanthobacter, autotrophicus Py2. This enzyme is an FAD-containing enzyme that is a, member of the NADPH:disulfide oxidoreductase (DSOR) family of enzymes that, include glutathione reductase, dihydrolipoamide dehydrogenase, trypanothione reductase, thioredoxin reductase, and mercuric reductase. In, contrast to the prototypical reactions catalyzed by members of the DSOR, family, the NADPH:2-ketopropyl-coenzyme M oxidoreductase/carboxylase, catalyzes the reductive cleavage of the thioether linkage of, 2-ketopropyl-coenzyme M, and the subsequent carboxylation of the, ketopropyl cleavage product, yielding the products acetoacetate and free, coenzyme M. The structure of 2-KPCC reveals a unique active site in, comparison to those of other members of the DSOR family of enzymes and, demonstrates how the enzyme architecture has been adapted for the more, sophisticated biochemical reaction. In addition, comparison of the, structures in the native state and in the presence of bound substrate, indicates the binding of the substrate 2-ketopropyl-coenzyme M induces a, conformational change resulting in the collapse of the substrate access, channel. The encapsulation of the substrate in this manner is reminiscent, of the conformational changes observed in the well-characterized, CO2-fixing enzyme ribulose 1,5-bisphosphate carboxylase/oxidase (Rubisco).
About this StructureAbout this Structure
1MOK is a Single protein structure of sequence from Xanthobacter sp. with FAD as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis for CO2 fixation by a novel member of the disulfide oxidoreductase family of enzymes, 2-ketopropyl-coenzyme M oxidoreductase/carboxylase., Nocek B, Jang SB, Jeong MS, Clark DD, Ensign SA, Peters JW, Biochemistry. 2002 Oct 29;41(43):12907-13. PMID:12390015
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