1lnu

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CRYSTAL STRUCTURE OF CLASS II MHC MOLECULE IAb BOUND TO EALPHA3K PEPTIDE

File:1lnu.gif


1lnu, resolution 2.50Å

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OverviewOverview

We have solved the crystal structure of the MHCII molecule, IA(b), containing an antigenic variant of the major IA(b)-binding peptide derived, from the MHCII IEalpha chain. The four MHC pockets at p1, p4, p6, and p9, that usually bind peptide side chains are largely empty because of, alanines in the peptide at these positions. The complex is nevertheless, very stable, apparently because of unique alternate interactions between, the IA(b) and peptide. In particular, there are multiple additional, hydrogen bonds between the N-terminal end of the peptide and the IA(b), alpha chain and an extensive hydrogen bond network involving an asparagine, at p7 position of the peptide and the IA(b) beta chain. By using knowledge, of the shape and size of the traditional side chain binding pockets and, the additional possible interactions, an IA(b) peptide-binding motif can, be deduced that agrees well with the sequences of known IA(b)-binding, peptides.

About this StructureAbout this Structure

1LNU is a Protein complex structure of sequences from Mus musculus with NAG and NDG as ligands. Full crystallographic information is available from OCA.

ReferenceReference

Alternate interactions define the binding of peptides to the MHC molecule IA(b)., Liu X, Dai S, Crawford F, Fruge R, Marrack P, Kappler J, Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8820-5. PMID:12084926

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