1lmo
THE CRYSTAL STRUCTURES OF THREE COMPLEXES BETWEEN CHITOOLIGOSACCHARIDES AND LYSOZYME FROM THE RAINBOW TROUT
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OverviewOverview
Like all c-type lysozymes, those from rainbow trout act as, 1,4-beta-acetyl-muramidases to destroy bacteria by cleaving the, polysaccharide chains of alternating N-acetylglucosamine (NAG) and, N-acetylmuramic acid (NAM) units in the cell walls. Lysozymes also, hydrolyse chitin, the analogous N-acetylglucosamine polymer. The rainbow, trout enzymes have been shown to be particularly effective in bacterial, defence. We have determined the crystal structures of three complexes, between rainbow trout lysozyme (RBTL) and the chito-oligosaccharides, (NAG)(2), (NAG)(3) and (NAG)(4) to resolutions of 1.8, 2.0 and 1.6 A, respectively. Crystals of these complexes were obtained by, co-crystallization, and intensity data were collected on a FAST area, detector system. Refinement and model building gave final R values of, 16.6, 15.9 and 16.5% for the di-, tri- and tetrasaccharide complexes, respectively. The results show that the chito-oligosaccharides bind to, sites A, B and C as previously observed for complexes between the hen, egg-white lysozyme (HEWL) and a variety of saccharides. The NAG ring in, site D is not bound so deeply and is only slightly distorted towards a, half-chair conformation as observed for the equivalent NAM residue in, HEWL. From our results, there is reason to question the position and the, degree of strain of the D saccharide and the mode of binding and, importance of two saccharides in sites E and F for correct orientation of, sugar D and effective hydrolysis of a productive substrate-lysozyme, complex. Simple model building study from our structures implies a, 'left-sided' binding mode of (NAG)(6) in the lower part of the active site, of RBTL.
About this StructureAbout this Structure
1LMO is a Single protein structure of sequence from Oncorhynchus mykiss. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.
ReferenceReference
Crystal structures of three complexes between chito-oligosaccharides and lysozyme from the rainbow trout. How distorted is the NAG sugar in site D?, Karlsen S, Hough E, Acta Crystallogr D Biol Crystallogr. 1995 Nov 1;51(Pt 6):962-78. PMID:15299765
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