1l4a
X-RAY STRUCTURE OF THE NEURONAL COMPLEXIN/SNARE COMPLEX FROM THE SQUID LOLIGO PEALEI
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OverviewOverview
Nerve terminals release neurotransmitters from vesicles into the synaptic, cleft upon transient increases in intracellular Ca(2+). This exocytotic, process requires the formation of trans SNARE complexes and is regulated, by accessory proteins including the complexins. Here we report the crystal, structure of a squid core complexin-SNARE complex at 2.95-A resolution. A, helical segment of complexin binds in anti-parallel fashion to the, four-helix bundle of the core SNARE complex and interacts at its C, terminus with syntaxin and synaptobrevin around the ionic zero layer of, the SNARE complex. We propose that this structure is part of a, multiprotein fusion machinery that regulates vesicle fusion at a late, pre-fusion stage. Accordingly, Ca(2+) may initiate membrane fusion by, acting directly or indirectly on complexin, thus allowing the, conformational transitions of the trans SNARE complex that are thought to, drive membrane fusion.
About this StructureAbout this Structure
1L4A is a Protein complex structure of sequences from Loligo plei. Full crystallographic information is available from OCA.
ReferenceReference
X-ray structure of a neuronal complexin-SNARE complex from squid., Bracher A, Kadlec J, Betz H, Weissenhorn W, J Biol Chem. 2002 Jul 19;277(29):26517-23. Epub 2002 May 9. PMID:12004067
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