1bj6
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1H NMR OF (12-53) NCP7/D(ACGCC) COMPLEX, 10 STRUCTURES
OverviewOverview
The nucleocapsid protein NCp7 of HIV-1 Mal contains two successive Zn, knuckles of the CX2CX4HX4C type and plays a major role in virion, morphogenesis, genomic RNA packaging and viral infectivity, mainly through, single-stranded nucleic acid binding. We report here the study by 1H 2D, NMR of the complex formed between the (12-53)NCp7, encompassing the two Zn, knuckles, and d(ACGCC), a deoxynucleotide sequence analog corresponding to, the shortest NCp7 binding site. Ten structures of the (12-53)NCp7/d(ACGCC), complex have been obtained from 607 NOE-derived distance constraints, 28, of which are intermolecular, and from molecular dynamics studies. The, oligonucleotide is almost perpendicular to the sequence linking the two Zn, knuckles. The Trp37 indole ring is inserted between the C2 and ... [(full description)]
About this StructureAbout this Structure
1BJ6 is a [Single protein] structure of sequence from [Human immunodeficiency virus 1] with ZN as [ligand]. Full crystallographic information is available from [OCA].
ReferenceReference
Structure of the complex between the HIV-1 nucleocapsid protein NCp7 and the single-stranded pentanucleotide d(ACGCC)., Morellet N, Demene H, Teilleux V, Huynh-Dinh T, de Rocquigny H, Fournie-Zaluski MC, Roques BP, J Mol Biol. 1998 Oct 23;283(2):419-34. PMID:9769215
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