1kp8
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Structural Basis for GroEL-assisted Protein Folding from the Crystal Structure of (GroEL-KMgATP)14 at 2.0 A Resolution
OverviewOverview
Nucleotide regulates the affinity of the bacterial chaperonin GroEL for, protein substrates. GroEL binds protein substrates with high affinity in, the absence of ATP and with low affinity in its presence. We report the, crystal structure of (GroEL-KMgATP)(14) refined to 2.0 A resolution in, which the ATP triphosphate moiety is directly coordinated by both K(+) and, Mg(2+). Upon the binding of KMgATP, we observe previously unnoticed domain, rotations and a 102 degrees rotation of the apical domain surface helix I., Two major consequences are a large lateral displacement of, and a dramatic, reduction of hydrophobicity in, the apical domain surface. These results, provide a basis for the nucleotide-dependent regulation of protein, substrate binding and suggest a mechanism for GroEL-assisted protein, folding by forced unfolding.
About this StructureAbout this Structure
1KP8 is a Single protein structure of sequence from Escherichia coli with K, SO4, MG and ATP as ligands. This structure superseeds the now removed PDB entry 1DER. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis for GroEL-assisted protein folding from the crystal structure of (GroEL-KMgATP)14 at 2.0A resolution., Wang J, Boisvert DC, J Mol Biol. 2003 Apr 4;327(4):843-55. PMID:12654267
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