1kkc
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Crystal structure of Aspergillus fumigatus MnSOD
OverviewOverview
Manganese superoxide dismutase (MnSOD) of Aspergillus fumigatus, a fungus, involved in many pulmonary complications, has been identified as, IgE-binding protein. It has been shown also that MnSODs from other, organisms, including human, are recognized by IgE Abs from individuals, sensitized to A. fumigatus MnSOD. Comparison of the fungal and the human, crystal structure should allow the identification of structural, similarities responsible for IgE-mediated cross-reactivity. The, three-dimensional structure of A. fumigatus MnSOD has been determined at, 2-A resolution by x-ray diffraction analysis. Crystals belonged to space, group P2(1)2(1)2(1) with unit cell dimensions of a = 65.88 A, b = 98.7 A, and c = 139.28 A. The structure was solved by molecular replacement using, the structure of the human MnSOD as a search model. The final refined, model included four chains of 199-200 amino acids, four manganese ions, and 745 water molecules, with a crystallographic R-factor of 19.4% and a, free R-factor of 23.3%. Like MnSODs of other eukaryotic organisms, A., fumigatus MnSOD forms a homotetramer with the manganese ions coordinated, by three histidines, one aspartic acid, and one water molecule. The fungal, and the human MnSOD share high similarity on the level of both primary and, tertiary structure. We identified conserved amino acids that are solvent, exposed in the fungal and the human crystal structure and are therefore, potentially involved in IgE-mediated cross-reactivity.
About this StructureAbout this Structure
1KKC is a Single protein structure of sequence from Aspergillus fumigatus with MN as ligand. Active as Superoxide dismutase, with EC number 1.15.1.1 Full crystallographic information is available from OCA.
ReferenceReference
Comparison of the crystal structures of the human manganese superoxide dismutase and the homologous Aspergillus fumigatus allergen at 2-A resolution., Fluckiger S, Mittl PR, Scapozza L, Fijten H, Folkers G, Grutter MG, Blaser K, Crameri R, J Immunol. 2002 Feb 1;168(3):1267-72. PMID:11801664
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