1kf5
Atomic Resolution Structure of RNase A at pH 7.1
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OverviewOverview
The diffraction pattern of protein crystals extending to atomic resolution, guarantees a very accurate picture of the molecular structure and enables, the study of subtle phenomena related to protein functionality. Six, structures of bovine pancreatic ribonuclease at the pH* values 5.2, 5.9, 6.3, 7.1, 8.0 and 8.8 and at resolution limits in the range 1.05-1.15A, have been refined. An overall description of the six structures and, several aspects, mainly regarding pH-triggered conformational changes, are, described here. Since subtle variations were expected, a thorough, validation assessment of the six refined models was first carried out., Some stereochemical parameters, such as the N[bond]C(alpha)[bond]C angle, and the pyramidalization at the carbonyl C atoms, indicate that the, standard target values and their weights typically used in refinement may, need revision. A detailed comparison of the six structures has provided, experimental evidence on the role of Lys41 in catalysis. Furthermore, insights are given into the structural effects related to the pH-dependent, binding of a sulfate anion, which mimics the phosphate group of RNA, in, the active site. Finally, the results support a number of thermodynamic, and kinetic experimental data concerning the role of the disulfide bridge, between Cys65 and Cys72 in the folding of RNase A.
About this StructureAbout this Structure
1KF5 is a Single protein structure of sequence from Bos taurus. Active as Pancreatic ribonuclease, with EC number 3.1.27.5 Full crystallographic information is available from OCA.
ReferenceReference
Atomic resolution structures of ribonuclease A at six pH values., Berisio R, Sica F, Lamzin VS, Wilson KS, Zagari A, Mazzarella L, Acta Crystallogr D Biol Crystallogr. 2002 Mar;58(Pt 3):441-50. Epub 2002, Feb 21. PMID:11856829
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