1k85
Solution structure of the fibronectin type III domain from Bacillus circulans WL-12 Chitinase A1.
|
OverviewOverview
Growing evidence suggests that horizontal gene transfer plays an integral, role in the evolution of bacterial genomes. One of the debated examples of, horizontal gene transfer from animal to prokaryote is the fibronectin type, III domain (FnIIID). Certain extracellular proteins of soil bacteria, contain an unusual cluster of FnIIIDs, which show sequence similarity to, those of animals and are likely to have been acquired horizontally from, animals. Here we report the solution structure of the FnIIID of chitinase, A1 from Bacillus circulans WL-12. To the best of our knowledge, this is, the first tertiary structure to be reported for an FnIIID from a bacterial, protein. The structure of the domain shows significant similarity to, FnIIIDs from animal proteins. Sequence comparisons with FnIIIDs from other, soil bacteria proteins show that the core-forming residues are highly, conserved and, thus, are under strong evolutionary pressure. Striking, similarities in the tertiary structures of bacterial FnIIIDs and their, mammalian counterparts may support the hypothesis that the evolution of, the FnIIID in bacterial carbohydrases occurred horizontally. The total, lack of surface-exposed aromatic residues also suggests that the role of, this FnIIID is different from those of other bacterial beta-sandwich, domains, which function as carbohydrate-binding modules.
About this StructureAbout this Structure
1K85 is a Single protein structure of sequence from Bacillus circulans. Active as Chitinase, with EC number 3.2.1.14 Full crystallographic information is available from OCA.
ReferenceReference
Solution structure of the fibronectin type III domain from Bacillus circulans WL-12 chitinase A1., Jee JG, Ikegami T, Hashimoto M, Kawabata T, Ikeguchi M, Watanabe T, Shirakawa M, J Biol Chem. 2002 Jan 11;277(2):1388-97. Epub 2001 Oct 12. PMID:11600504
Page seeded by OCA on Tue Nov 20 18:56:21 2007