1jta
Crystal Structure of Pectate Lyase A (C2 form)
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OverviewOverview
Pectate lyase A is a virulence factor secreted by the plant-pathogenic, bacteria Erwinia chrysanthemi. The enzyme cleaves the glycosidic bond of, pectate polymers by a calcium-dependent beta-elimination mechanism. The, crystal structure of pectate lyase A from E. chrysanthemi EC16 has been, determined in two crystal forms, monoclinic C2 to 1.8 A and rhombohedral, R3 to 2.1 A. The protein structure is compared with two other pectate, lyase isoforms from E. chrysanthemi EC16, pectate lyase C and pectate, lyase E. Pectate lyase A is unique as it is the only acidic pectate lyase, and has end products that are significantly more varied in length in, comparison to those of the other four major pectate lyase isozymes., Differences and similarities in polypeptide trace, size and volume of the, active-site groove and surface electrostatics are discussed.
About this StructureAbout this Structure
1JTA is a Single protein structure of sequence from Erwinia chrysanthemi with SO4 as ligand. Active as Pectate lyase, with EC number 4.2.2.2 Full crystallographic information is available from OCA.
ReferenceReference
Structure of pectate lyase A: comparison to other isoforms., Thomas LM, Doan CN, Oliver RL, Yoder MD, Acta Crystallogr D Biol Crystallogr. 2002 Jun;58(Pt 6 Pt 2):1008-15. Epub, 2002 May 29. PMID:12037303
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