1jp8
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Sperm Whale met-Myoglobin (room temperature; high pressure)
OverviewOverview
Pressures in the 100 MPa range are known to have an enormous number of, effects on the action of proteins, but straightforward means for, determining the structural basis of these effects have been lacking. Here, crystallography has been used to probe effects of pressure on sperm whale, myoglobin structure. A comparison of pressure effects with those seen at, low pH suggests that structural changes under pressure are interpretable, as a shift in the populations of conformational substates. Furthermore, a, novel high-pressure protein crystal-cooling method has been used to show, low-temperature metastability, providing an alternative to room, temperature, beryllium pressure cell-based techniques. The change in, protein structure due to pressure is not purely compressive and involves, conformational changes important to protein activity. Correlation with, low-pH structures suggests observed structural changes are associated with, global conformational substates. Methods developed here open up a direct, avenue for exploration of the effects of pressure on proteins.
About this StructureAbout this Structure
1JP8 is a Single protein structure of sequence from Physeter catodon with SO4 and HEM as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Probing substates in sperm whale myoglobin using high-pressure crystallography., Urayama P, Phillips GN Jr, Gruner SM, Structure. 2002 Jan;10(1):51-60. PMID:11796110
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